| 1 | |  Chattanooga FC | 6 | 5 | 1 | 0 | 13 | 5 | 8 | 17 | G03:00 | 08/03/25 | R 1  Inter Miami CF II 1 Chattanooga FC 2 G22:30 | 30/03/25 | R 1 Atlanta United 2 (3) 2 Chattanooga FC (4) 2 G22:00 | 13/04/25 | R 1 Carolina Core FC 1 Chattanooga FC 4 G02:00 | 20/04/25 | R 1 Chattanooga FC 2 Crown Legacy FC 1 |
| 2 | |  Minnesota United FC 2 | 4 | 4 | 0 | 0 | 10 | 3 | 7 | 12 | G04:00 | 31/03/25 | R 1 Houston Dynamo 2 1 Minnesota United FC 2 3 G03:00 | 25/04/25 | R 1 Colorado Rapids 2 0 Minnesota United FC 2 2 |
| 3 | |  Houston Dynamo 2 | 6 | 4 | 0 | 2 | 13 | 7 | 6 | 12 | G04:30 | 09/03/25 | R 1 Houston Dynamo 2 3 Sporting Kansas City II 1 M05:00 | 27/03/25 | R 1 Los Angeles Galaxy II 1 Houston Dynamo 2 0 M04:00 | 31/03/25 | R 1 Houston Dynamo 2 1 Minnesota United FC 2 3 G02:00 | 07/04/25 | R 1 Houston Dynamo 2 1 Los Angeles FC 2 0 G04:00 | 24/04/25 | R 1 Vancouver Whitecaps FC II 1 Houston Dynamo 2 3 |
| 4 | |  Los Angeles Galaxy II | 6 | 4 | 0 | 2 | 12 | 10 | 2 | 12 | G06:00 | 08/03/25 | R 1 Los Angeles Galaxy II 3 Tacoma Defiance 2 M05:00 | 15/03/25 | R 1 Los Angeles FC 2 3 Los Angeles Galaxy II 1 G05:00 | 27/03/25 | R 1 Los Angeles Galaxy II 1 Houston Dynamo 2 0 G03:00 | 07/04/25 | R 1 Real Monarchs SLC 1 Los Angeles Galaxy II 2 G03:00 | 14/04/25 | R 1 Los Angeles Galaxy II 3 Saint Louis City SC 2 1 M05:00 | 24/04/25 | R 1 Los Angeles Galaxy II 2 Portland Timbers II 3 |
| 5 | |  North Texas SC | 8 | 3 | 2 | 3 | 14 | 18 | -4 | 12 | M03:30 | 10/03/25 | R 1 North Texas SC 0 Saint Louis City SC 2 3 G03:30 | 15/03/25 | R 1 North Texas SC 3 Vancouver Whitecaps FC II 2 M03:45 | 29/03/25 | R 1 North Texas SC (4) 3 Sporting Kansas City II (5) 3 G03:30 | 12/04/25 | R 1 Austin FC II (2) 1 North Texas SC (4) 1 M03:30 | 21/04/25 | R 1 North Texas SC 2 Colorado Rapids 2 3 G03:30 | 24/04/25 | R 1 North Texas SC 2 San Jose Earthquakes II 0 |
| 6 | |  Saint Louis City SC 2 | 6 | 3 | 1 | 2 | 7 | 4 | 3 | 11 | G03:30 | 10/03/25 | R 1 North Texas SC 0 Saint Louis City SC 2 3 G00:30 | 31/03/25 | R 1 Saint Louis City SC 2 (5) 0 Austin FC II (4) 0 M00:00 | 07/04/25 | R 1 Saint Louis City SC 2 0 Colorado Rapids 2 1 M03:00 | 14/04/25 | R 1 Los Angeles Galaxy II 3 Saint Louis City SC 2 1 |
| 7 | |  Orlando City B | 6 | 3 | 1 | 2 | 8 | 8 | 0 | 11 | G02:00 | 27/03/25 | R 1 Crown Legacy FC 0 Orlando City B 1 M02:00 | 16/04/25 | R 1 Inter Miami CF II 3 Orlando City B 2 |
| 8 | |  New York Red Bulls II | 5 | 3 | 1 | 1 | 9 | 8 | 1 | 10 | G03:30 | 08/03/25 | R 1 Atlanta United 2 0 New York Red Bulls II 1 M02:00 | 18/03/25 | R 1 Philadelphia Union II (3) 1 New York Red Bulls II (1) 1 G21:00 | 30/03/25 | R 1 New York Red Bulls II 3 New York City FC II 2 G00:00 | 07/04/25 | R 1 New York Red Bulls II 3 Chicago Fire FC II 2 M01:00 | 21/04/25 | R 1 FC Cincinnati 2 3 New York Red Bulls II 1 |
| 9 | |  Colorado Rapids 2 | 6 | 3 | 1 | 2 | 10 | 11 | -1 | 10 | M03:00 | 24/03/25 | R 1 Real Monarchs SLC (7) 2 Colorado Rapids 2 (6) 2 G00:00 | 07/04/25 | R 1 Saint Louis City SC 2 0 Colorado Rapids 2 1 G23:00 | 13/04/25 | R 1 Portland Timbers II 1 Colorado Rapids 2 2 G03:30 | 21/04/25 | R 1 North Texas SC 2 Colorado Rapids 2 3 M03:00 | 25/04/25 | R 1 Colorado Rapids 2 0 Minnesota United FC 2 2 |
| 10 | |  Toronto FC II | 6 | 3 | 1 | 2 | 5 | 6 | -1 | 10 | M03:00 | 14/03/25 | R 1 Chicago Fire FC II 1 Toronto FC II 0 G02:00 | 11/04/25 | R 1 Inter Miami CF II 0 Toronto FC II 1 M02:00 | 19/04/25 | R 1 Toronto FC II (1) 1 Carolina Core FC (4) 1 |
| 11 | |  Philadelphia Union II | 5 | 2 | 3 | 0 | 12 | 5 | 7 | 10 | G02:00 | 18/03/25 | R 1 Philadelphia Union II (3) 1 New York Red Bulls II (1) 1 M03:00 | 29/03/25 | R 1 Huntsville City FC (4) 2 Philadelphia Union II (3) 2 G02:00 | 11/04/25 | R 1 Philadelphia Union II 5 Atlanta United 2 1 |
| 11 | |  Huntsville City FC | 5 | 2 | 2 | 1 | 12 | 5 | 7 | 10 | G03:00 | 29/03/25 | R 1 Huntsville City FC (4) 2 Philadelphia Union II (3) 2 G02:00 | 14/04/25 | R 1 Crown Legacy FC (3) 1 Huntsville City FC (4) 1 G02:00 | 21/04/25 | R 1 Huntsville City FC 5 Chicago Fire FC II 0 |
| 13 | |  New England Revolution II | 5 | 2 | 2 | 1 | 8 | 3 | 5 | 10 | G00:00 | 14/03/25 | R 1 New England Revolution II 2 FC Cincinnati 2 0 |
| 14 | |  Tacoma Defiance | 5 | 3 | 0 | 2 | 16 | 11 | 5 | 9 | M06:00 | 08/03/25 | R 1 Los Angeles Galaxy II 3 Tacoma Defiance 2 G05:00 | 15/03/25 | R 1 Tacoma Defiance 3 San Jose Earthquakes II 1 G05:00 | 07/04/25 | R 1 Tacoma Defiance 5 Vancouver Whitecaps FC II 3 M05:00 | 11/04/25 | R 1 Los Angeles FC 2 3 Tacoma Defiance 2 |
| 15 | |  Los Angeles FC 2 | 5 | 2 | 2 | 1 | 10 | 8 | 2 | 9 | M05:00 | 10/03/25 | R 1 Los Angeles FC 2 (2) 3 Vancouver Whitecaps FC II (4) 3 G05:00 | 15/03/25 | R 1 Los Angeles FC 2 3 Los Angeles Galaxy II 1 M02:00 | 07/04/25 | R 1 Houston Dynamo 2 1 Los Angeles FC 2 0 G05:00 | 11/04/25 | R 1 Los Angeles FC 2 3 Tacoma Defiance 2 |
| 16 | |  Real Monarchs SLC | 5 | 2 | 2 | 1 | 9 | 8 | 1 | 9 | G05:30 | 16/03/25 | R 1 Portland Timbers II 2 Real Monarchs SLC 3 G03:00 | 24/03/25 | R 1 Real Monarchs SLC (7) 2 Colorado Rapids 2 (6) 2 M03:00 | 07/04/25 | R 1 Real Monarchs SLC 1 Los Angeles Galaxy II 2 G03:00 | 11/04/25 | R 1 Real Monarchs SLC 2 San Jose Earthquakes II 1 |
| 17 | |  New York City FC II | 7 | 2 | 2 | 3 | 11 | 17 | -6 | 9 | G03:00 | 08/03/25 | R 1 Crown Legacy FC (4) 2 New York City FC II (5) 2 G02:30 | 25/03/25 | R 1 New York City FC II 2 Carolina Core FC 1 M21:00 | 30/03/25 | R 1 New York Red Bulls II 3 New York City FC II 2 |
| 18 | |  Carolina Core FC | 6 | 1 | 3 | 2 | 11 | 10 | 1 | 8 | G02:00 | 14/03/25 | R 1 Inter Miami CF II (2) 1 Carolina Core FC (4) 1 M02:30 | 25/03/25 | R 1 New York City FC II 2 Carolina Core FC 1 M22:00 | 13/04/25 | R 1 Carolina Core FC 1 Chattanooga FC 4 G02:00 | 19/04/25 | R 1 Toronto FC II (1) 1 Carolina Core FC (4) 1 |
| 19 | |  Inter Miami CF II | 6 | 2 | 1 | 3 | 11 | 11 | 0 | 7 | M03:00 | 08/03/25 | R 1 Inter Miami CF II 1 Chattanooga FC 2 M02:00 | 14/03/25 | R 1 Inter Miami CF II (2) 1 Carolina Core FC (4) 1 M02:00 | 11/04/25 | R 1 Inter Miami CF II 0 Toronto FC II 1 G02:00 | 16/04/25 | R 1 Inter Miami CF II 3 Orlando City B 2 |
| 20 | |  Vancouver Whitecaps FC II | 6 | 1 | 2 | 3 | 14 | 15 | -1 | 7 | G05:00 | 10/03/25 | R 1 Los Angeles FC 2 (2) 3 Vancouver Whitecaps FC II (4) 3 M03:30 | 15/03/25 | R 1 North Texas SC 3 Vancouver Whitecaps FC II 2 M05:00 | 07/04/25 | R 1 Tacoma Defiance 5 Vancouver Whitecaps FC II 3 G02:00 | 20/04/25 | R 1 Vancouver Whitecaps FC II 4 Austin FC II 0 M04:00 | 24/04/25 | R 1 Vancouver Whitecaps FC II 1 Houston Dynamo 2 3 |
| 21 | |  Atlanta United 2 | 6 | 1 | 3 | 2 | 10 | 14 | -4 | 7 | M03:30 | 08/03/25 | R 1 Atlanta United 2 0 New York Red Bulls II 1 M22:30 | 30/03/25 | R 1 Atlanta United 2 (3) 2 Chattanooga FC (4) 2 M02:00 | 11/04/25 | R 1 Philadelphia Union II 5 Atlanta United 2 1 |
| 22 | |  Chicago Fire FC II | 6 | 2 | 0 | 4 | 6 | 15 | -9 | 6 | G03:00 | 14/03/25 | R 1 Chicago Fire FC II 1 Toronto FC II 0 G23:00 | 30/03/25 | R 1 FC Cincinnati 2 1 Chicago Fire FC II 2 M00:00 | 07/04/25 | R 1 New York Red Bulls II 3 Chicago Fire FC II 2 M02:00 | 21/04/25 | R 1 Huntsville City FC 5 Chicago Fire FC II 0 |
| 23 | |  Portland Timbers II | 6 | 1 | 2 | 3 | 9 | 11 | -2 | 6 | M05:30 | 16/03/25 | R 1 Portland Timbers II 2 Real Monarchs SLC 3 G05:30 | 27/03/25 | R 1 Portland Timbers II (4) 1 San Jose Earthquakes II (3) 1 M23:00 | 13/04/25 | R 1 Portland Timbers II 1 Colorado Rapids 2 2 G05:00 | 24/04/25 | R 1 Los Angeles Galaxy II 2 Portland Timbers II 3 |
| 24 | |  FC Cincinnati 2 | 5 | 1 | 1 | 3 | 6 | 8 | -2 | 5 | M00:00 | 14/03/25 | R 1 New England Revolution II 2 FC Cincinnati 2 0 M23:00 | 30/03/25 | R 1 FC Cincinnati 2 1 Chicago Fire FC II 2 G01:00 | 21/04/25 | R 1 FC Cincinnati 2 3 New York Red Bulls II 1 |
| 25 | |  Austin FC II | 5 | 1 | 2 | 2 | 5 | 8 | -3 | 5 | M00:30 | 31/03/25 | R 1 Saint Louis City SC 2 (5) 0 Austin FC II (4) 0 M03:30 | 12/04/25 | R 1 Austin FC II (2) 1 North Texas SC (4) 1 M02:00 | 20/04/25 | R 1 Vancouver Whitecaps FC II 4 Austin FC II 0 |
| 26 | |  Crown Legacy FC | 5 | 0 | 3 | 2 | 5 | 7 | -2 | 4 | M03:00 | 08/03/25 | R 1 Crown Legacy FC (4) 2 New York City FC II (5) 2 M02:00 | 27/03/25 | R 1 Crown Legacy FC 0 Orlando City B 1 G01:00 | 07/04/25 | R 1 Columbus Crew 2 (3) 1 Crown Legacy FC (4) 1 M02:00 | 14/04/25 | R 1 Crown Legacy FC (3) 1 Huntsville City FC (4) 1 M02:00 | 20/04/25 | R 1 Chattanooga FC 2 Crown Legacy FC 1 |
| 27 | |  Columbus Crew 2 | 5 | 0 | 2 | 3 | 6 | 11 | -5 | 2 | M01:00 | 07/04/25 | R 1 Columbus Crew 2 (3) 1 Crown Legacy FC (4) 1 |
| 28 | |  Sporting Kansas City II | 6 | 0 | 1 | 5 | 7 | 17 | -10 | 2 | M04:30 | 09/03/25 | R 1 Houston Dynamo 2 3 Sporting Kansas City II 1 G03:45 | 29/03/25 | R 1 North Texas SC (4) 3 Sporting Kansas City II (5) 3 |
| 29 | |  San Jose Earthquakes II | 4 | 0 | 1 | 3 | 3 | 8 | -5 | 1 | M05:00 | 15/03/25 | R 1 Tacoma Defiance 3 San Jose Earthquakes II 1 M05:30 | 27/03/25 | R 1 Portland Timbers II (4) 1 San Jose Earthquakes II (3) 1 M03:00 | 11/04/25 | R 1 Real Monarchs SLC 2 San Jose Earthquakes II 1 M03:30 | 24/04/25 | R 1 North Texas SC 2 San Jose Earthquakes II 0 |
